Functional Carbohydrate Development: From Byproduct Recovery to Substrate-Microbial Interactions

Dates: April 21, 2017

Friday, April 21, 2017
1:30-2:30 pm
Morgan Conference Room

Ming-Hsu Chen

Department of Agricultural and Biological Engineering

University of Illinois at Urbana-Champaign

chen289@illinois.edu

Miscanthus x giganteus (MxG) is considered a potential bioenergy crop due to its high yield and ecological properties. Here MxG was explored as a feedstock for production of xylooligosaccharides (XOS). Due to their prebiotic functionality and other health promoting effects, XOS can be a value added coproduct for the lignocellulosic ethanol industry. XOS was extracted from MxG by autohydrolysis at 160-200°C for 0 to 60 min. The released XOS was recovered using activated carbon coupled with ethanol/water elution. The ethanol eluted XOS fractions were combined and further purified using ion exchange resins. Intermediate and final XOS were evaluated for yield, sugar composition, and degree of polymerization. The end product was evaluated through in vitro fermentation cultures of representative gut bacteria. Fermentation was followed by measuring growth and production of organic acids. As a final test, purified MxG XOS were cultured with human fecal microbiota to simulate digestion in the colon. Culture pH and fermentation products were measured after 12 hr. The qPCR was adopted to enumerate bacteria populations. With MxG XOS used as the substrate, the Bifidobacteria spp. and Lactobacillus spp. increased and Clostridium perfringens decreased in comparison to control cultures. In summary, a process was developed for production and recovery of XOS from MxG that function as a prebiotic.

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